Volume 40 ·  Issue 11 ·  2004

Angle-closure glaucoma is the main type of glaucoma in China, and its blindness rate is 10 times that of open-angle glaucoma [1, 2]. Early intervention for angle-closure glaucoma can effectively prevent the increase of intraocular pressure caused by angle closure, and filtration surgery has good therapeutic effect for middle and late angle-closure glaucoma. Therefore, angle-closure glaucoma, as a kind of preventable and treatable eye disease, will gradually become the focus of blindness prevention in China in the new century with the reduction of the number of curable eye diseases such as cataract and corneal disease. In the current prevention and treatment of angle-closure glaucoma in China, some basic problems are often neglected, which seriously affects the overall diagnosis and treatment level and prevention and treatment effect of angle-closure glaucoma in China, and should be paid great attention by the majority of ophthalmologists.

Cataract extraction combined with intraocular lens implantation replaces about 5.5 mm thick human lens with less than 1.0 mm thick intraocular lens, which can significantly deepen the depth of the central anterior chamber and shift the contact plane between the pupil edge and the lens [1], thus relieving the pupil block. In recent years, ophthalmologists at home and abroad have found that simple cataract surgery has the effect of controlling intraocular pressure on partial angle-closure glaucoma in the staged treatment of cataract extraction and anti-glaucoma surgery, so they have put forward the treatment method of simple cataract surgery for angle-closure glaucoma, thus expanding the application scope of lens extraction. This method can not only simplify the surgical procedure, but also improve the visual condition of patients. However, the mechanism of lowering intraocular pressure of simple cataract surgery for angle-closure glaucoma has not been completely elucidated at present, the therapeutic effect is different, and the surgical indication is unclear. Therefore, this treatment choice has become the focus of ophthalmology at home and abroad in recent years, and the author expounds his own views on this.

Objective to investigate the prevalence of primary open-angle glaucoma (POAG) in people over 40 years old in Beijing and evaluate the screening methods of POAG. Methods From June to October 2001, a door-to-door registration method was adopted to investigate the epidemiology of ophthalmopathy in people over 40 years old in five communities in the north of Beijing and three natural villages in the southern end of Beijing. The on-site examination items of glaucoma screening included corrected visual acuity, optometry, frequency doubling perimetry, non-contact tonometer, Van Herick method to assess the peripheral anterior chamber depth and fundus photography. All patients with suspicious glaucoma and glaucoma underwent standard glaucoma examination, including re-examination of intraocular pressure, gonioscopy, threshold visual field examination with Octopus automatic perimetry and fundus stereo photography. Results The number of people examined in rural and urban areas was 1,980 and 2,471, respectively, with a total of 4,451 people. The response rates were 79.58% and 87.13%, respectively. The prevalence rates of POAG in rural and urban areas were 1.97% and 2.07%, respectively, The prevalence of POAG in rural and urban women was 1.04% and 1.42%, respectively. The prevalence of POAG increased with age, and the rising curve changed exponentially. About 50% of the diagnosed glaucoma patients had initial IOP<21 mm Hg (1 mm Hg =0.133 kPa), and the newly found POAG accounted for 92.30% and 87.30% in rural and urban areas, respectively; The monocular blindness rate of POAG is 15.40% and 10.90% in rural and urban areas, respectively.Conclusion The comprehensive evaluation of optic nerve and retinal nerve fiber layers by fundus photography shows that the prevalence of glaucoma detected is higher than that of previous domestic studies, which is basically consistent with the recent studies in Asia and other countries.The possible reason why the prevalence of POAG in rural women is lower than that in urban areas is that the anterior chamber of rural women is shallow.

Objective to evaluate the long-term efficacy of trabeculotomy in the treatment of primary congenital glaucoma.Methods the long-term efficacy of 22 patients (33 eyes) with primary congenital glaucoma who underwent first-time trabeculotomy was retrospectively analyzed.The criteria of successful surgery were as follows: the intraocular pressure ≤21 mm Hg (1 mm Hg =0.133 kPa), the cornea was clear, the transverse diameter of the cornea and the cup/disc ratio were decreased or not progressed.Results the follow-up time of the patients was more than 1 year, the average follow-up time was (54.2 ± 33.2) months, and the follow-up time was more than 3 years, accounting for 72.7%. The postoperative mean intraocular pressure was (16.1 ± 6.2) mm Hg, which was significantly lower than that of the preoperative intraocular pressure (29.9 ± 11.7) mm Hg (P<0.001); The average transverse corneal diameter (12.9±1.0) mm after operation was significantly reduced compared with that before operation (13.7±1.4) mm (P =0.002), and the average cup/disc ratio was not significantly different before and after operation (P =0.148). The cup/disc ratio was significantly reduced in successful patients (0.7±0.2 before operation and 0.6±0.3 after operation, P =0.007). According to the above criteria, the success rate of operation at 1, 3 and 5 years was 97.0%, 93.2% and 74.5% respectively (Kaplan-Meier survival analysis). Thirteen eyes (39.6%) underwent more than two operations, and the success rate of reoperation was 69.2%. The corrected visual acuity ≥0.4 accounted for 41.6%, 0.1~0.3 accounted for 29.2%, and<0.1 accounted for 29.2%. The cup/disc ratio>0.8 was the risk factor of low visual acuity (linear regression analysis, P =0.013). In this group, 18 eyes underwent visual field examination, and 14 eyes (77.7%) had glaucomatous visual field defects. The degree of visual field defects: early damage accounted for 42.8%, middle and late damage accounted for 28.6% respectively. Intraoperative anterior chamber hemorrhage was observed in 14 eyes, accounting for 42.4%, and the vast majority of eyes (71.4%) had hyphema absorption 1 d after operation. All patients had no other complications. Whether anterior chamber hemorrhage was not related to the success rate of operation (linear regression analysis, P =0.795). Conclusion Trabeculotomy for primary congenital glaucoma has a high success rate, few complications, and good long-term efficacy.

Objective to evaluate the diagnostic value of retinal tomography in glaucomatous optic neuropathy. Methods Heidelberg retinal tomography (HRT) was used to detect the topographic parameters of the optic head in 116 normal subjects (116 eyes) and 63 patients (99 eyes) with primary open angle glaucoma (POAG). The parameters of papilla topography were analyzed step by step between normal subjects and POAG patients, and the discriminant function was established. The sensitive parameters for diagnosis of POAG were screened out and analyzed by substitution. Results Optic disc area (DA), optic cup area (CA), cup/disc area (C/DAR), disc edge area (RA), height change of papilla contour line (HVC), optic cup volume (CV), disc edge volume (RV), mean optic cup depth (MCD), maximum optic cup depth (MxCD), optic cup morphometry (CSM), mean retinal nerve fiber layer thickness (mRNFLT) and retinal nerve fiber layer cross-sectional area (RNFLA) of POAG patients were significantly different from normal subjects (P<0.001); The parameters of optic disk topography sensitive to POAG diagnosis are C/DAR, RA, CSM, CV and MxCD; The discriminant function formula is Y = -2.083-4.833× C/DAR-20.379× CSM +2.035× RA +3.955× CV-3.701× MxCD; The sensitivity, specificity and coincidence rate of diagnosis of POAG were 81.8%, 86.2% and 84.2%, respectively, which were comparable to HRT automatic analysis program.Conclusion The topography of the optic papilla of POAG patients was quite different from that of normal subjects; The diagnostic efficiency of the discriminant function established by using human data in China is comparable to that of the HRT automatic analysis program, which shows that the HRT automatic analysis program is suitable for the diagnosis of POAG in China to a certain extent.

Objective to investigate the safety value of residual corneal stromal bed thickness after excimer laser in situ keratomileusis (LASIK) and the stabilization time of postoperative posterior corneal surface curvature. Methods 117 cases (227 eyes) were randomly selected from patients undergoing LASIK in 2000. The results of ORBSCAN Ⅱ posterior corneal surface curvature measurement were compared and analyzed before and at 1 month, 3 months, 6 months, 1 year and 2 years after LASIK. Results the center of the posterior corneal surface was bulged forward to varying degrees in the early stage of LASIK, and the thinner the corneal stromal bed (<There was no significant difference in the curvature of the posterior corneal surface between 2 years and 1 year after surgery (P>0.05). Conclusion Postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative postoperative

Objective to investigate the long-term efficacy of amniotic membrane transplantation in reconstructing the defective conjunctival surface after blepharoplasty, and to explore the related influencing factors.Methods 51 patients (55 eyes) with blepharoplasty due to old chemical injury, thermal burn or Stevens-Johnson syndrome were treated with blepharoplasty combined with amniotic membrane transplantation.Among 33 eyes with old chemical injury, 10 eyes underwent surgery from 5.0 to 11.0 months after burn, and 23 eyes underwent surgery from 1.0 to 8.0 years after burn. Results 51 patients were followed up for 26.0 to 30.0 months, with an average of (27.4±2.6) months.All amniotic membrane grafts transplanted on the conjunctival surface did not ulcerate or dissolve in the early postoperative stage, and the peripheral alignment was good. 31 eyes (56.4%) formed a sufficiently deep fornix and recovered eye movement function. 9 eyes (16.4%) had partial blepharoplasty, with slight eye movement limitation, but its area was much smaller than that before surgery. 15 eyes (27.3%) had moderate or above blepharoplasty after surgery, Among them, 8 eyes with total conjunctival sac formation due to palpebral atresia had severe symblepharoplasty in the conjunctival sac again within 1 month after operation, and 4 eyes with moderate symblepharoplasty due to Stevens-Johnson syndrome had completely released adhesion after operation. Patients with different degrees of symblepharoplasty had different effects after amniotic membrane transplantation, and the more severe the adhesion, the worse the effect (P =0.000). There was a significant difference in prognostic effect between surgery performed after (7.2±1.4) months of ocular surface chemical burn and amniotic membrane transplantation performed more than 1 year after burn (P =0.039). There was a significant difference in prognostic effect between different tear secretion groups (P =0.000). The more severe the dry eye syndrome, the worse the effect. Conclusion Amniotic membrane can effectively reconstruct the defective conjunctival surface after separation of symblepharoplasty, and some eyes may need multiple surgeries to obtain better results. The degree of symblepharoplasty and dry eye before operation and whether some conjunctival epithelium remains will affect the long-term efficacy of amniotic membrane transplantation to reconstruct the conjunctival surface.

Objective to investigate the influence of preoperative diagnosis and perioperative drug therapy on postoperative recurrence of severe acanthamoeba keratitis treated by penetrating keratoplasty. Methods 18 cases of acanthamoeba keratitis diagnosed and treated from 1996 to 2002 were analyzed. 12 cases were diagnosed before operation and 6 cases were misdiagnosed. The diameter of corneal infection was greater than 7 mm. During the treatment, all 18 patients underwent penetrating keratoplasty (PKP) due to the aggravation of their disease. According to the preoperative diagnosis, the confirmed patients were given anti-amoeba therapy, while the misdiagnosed patients were given anti-viral, fungal and bacterial therapy before operation. Seven patients (2 cases were diagnosed, 3 cases were misdiagnosed as viral keratitis and 2 cases were misdiagnosed as bacterial keratitis) received systemic and local glucocorticoid drugs in the early postoperative period. Results the average follow-up time was 10 months. There was no recurrence in 13 cases (72%), of which 11 cases were diagnosed before operation and 2 cases were misdiagnosed. Five patients (28%) had postoperative recurrence.Seven patients received continuous glucocorticoid therapy after surgery, and four of them had recurrence.The ratio ratios of misdiagnosis and early postoperative continuous glucocorticoid use on recurrence were 22 (P =0.021) and 13 (P =0.047), respectively. Conclusion PKP is an effective method for the treatment of severe Acanthamoeba keratitis that cannot be controlled by drugs. Preoperative definite diagnosis, perioperative anti-amoeba therapy and early avoidance of continuous use of glucocorticoids can reduce the recurrence rate of Acanthamoeba keratitis after operation.

Objective to investigate the possible role of proto-oncogenes mdm2 and p53 in the pathogenesis of orbital rhabdomyosarcoma (ORMS), and to demonstrate the feasibility of mdm2 and p53 as markers of biological characteristics and prognostic indexes of ORMS. Methods The expression of mdm2, p53 and Ki-67 protein was detected by immunohistochemistry in 31 ORMS specimens. The expression of Ki-67 protein was used as a proliferation index to reflect the proliferation status of tumor cells. The expression of mdm2 and p53 gene at mRNA level in 31 ORMS specimens was detected by in situ hybridization method. The relationship between the expression of mdm2 and p53 protein and clinical histopathological indexes such as age, sex, pathological type, differentiation degree and proliferation degree was analyzed. Results In 31 ORMS, the positive rate of mdm2 and p53 protein expression was 77.4% (24/31) and 71.0% (22/31), respectively, and the positive rate of mdm2 and p53 protein co-expression was 61.3%. There was good agreement between the expression of mdm2 and p53 protein level and their respective mRNA level The difference was significant (P =0.007, 0.009). (2) According to the expression of Ki-67, they were divided into high and low proliferation groups. The positive rate of p53 protein expression in high proliferation group was higher than that in low proliferation group, and the difference was significant (P =0.044). There was no significant correlation between the expression of mdm2 and p53 protein and other clinical pathological indexes (P>0.05). Conclusion mdm2 and p53 are closely related to the differentiation and proliferation of ORMS, and the co-expression of mdm2 and p53 suggests that mdm2 may bind to p53 to regulate its activity. The abnormal expression of mdm2 and p53 may lead to cell atypia and hyperproliferation, which plays an important role in the occurrence and development of ORMS. mdm2 and p53 can reflect the differentiation and proliferation characteristics of tumor to some extent, and may be one of the prognostic indexes of tumor.

Objective to investigate the effects of gas perfusion on the morphological structure and physiological function of the retina in rabbits during vitrectomy, in order to clarify the mechanism of visual function impairment after vitrectomy. Methods 24 gray rabbits were randomly divided into 3 groups to undergo vitrectomy. Group A and B underwent gas-liquid exchange under gas perfusion pressure of 25 and 40 mm Hg (1 mm Hg =0.133 kpa), respectively, while the control group did not undergo gas-liquid exchange. Clinical observation and electroretinogram examination were performed after operation, and the eyeball was removed after 6 weeks and examined by light microscope and transmission electron microscope. Results There were no obvious abnormal signs in the eyes of each group. 1 day after operation, the bA ratio of electroretinogram in group A and B was significantly different from that of the control group, but the bA ratio in group B was still significantly reduced at 6 weeks after operation. Under light microscope, the contralateral retina structure was disordered with partial tissue loss after perfusion in group B, and the retina thickness was significantly different from that of the control group. The thickness of outer retina decreased more obviously.The electron microscope showed that there was obvious nerve fiber and photoreceptor cell damage in the contralateral retina of rabbits in group B after perfusion, while the injury was slight in rabbits in group A, and there was no abnormality in the retina of rabbits in each group.Conclusion Gas perfusion during vitrectomy can cause irreversible damage to the retina of rabbits, and gas perfusion may be one of the main causes of visual function damage after vitrectomy.

Objective to study the temporal and spatial distribution of markers and expression of neural stem cells in the retina. Methods The expression of Nestin, Vimentin, GFAP and NF in the retina of rats from newborn to 12 months of age was detected by immunohistochemical staining. Results the differentiation of retinal structure in rats could not be completed until 2 weeks after birth. The expression level of Nestin was significantly reduced in nerve cells, glial cells and vascular endothelial cells in the retina within 2 weeks. After 2 weeks, the expression level of Nestin was significantly reduced. At 4 weeks, the expression level of Nestin was completely negative. However, the expression of Nestin continued to be high in a few processes of ciliary body. In adulthood, the positive cells were mainly pigment epithelial cells. In addition, Nestin positive cells were scattered in the optic nerve of adult rats. Vimentin was strongly positive in glial cells and pigment-free epithelial cells of ciliary body. We also found that Vimentin was expressed in Müller cells and amacrine cells within 4 weeks. GFAP and NF were expressed only in the axons of mature glial cells and ganglion cells. Conclusion Nestin can be used as a marker of retinal neural stem cells, which are distributed in a few ciliary processes and optic nerves in adulthood.

Objective to investigate the role of angiotensin Ⅱ (ANG Ⅱ) in the occurrence and development of diabetic retinopathy (DR) and the preventive and therapeutic effects of captopril and valsartan on DR. Methods 72 3-week-old male C57BL/ksj db/ + mice were randomly divided into 4 groups. After 4 weeks of high-fat diet, 3 groups were given intraperitoneal injection of streptozotocin (STZ) to induce diabetes. Two intervention groups were given valsartan 40 mg/kg body weight or captopril 12.5 mg/kg body weight daily by gavage, while the control group and diabetic group were given equal amount of normal saline by gavage. At 4, 8 and 12 weeks after intervention, 6 eyeballs in each group were removed and stained by immunohistochemistry. The mean gray scale and area density of Ang Ⅱ and vascular endothelial growth factor (VEGF) in the retinal ganglion cell layer were analyzed by computer-image analyzer. Results With the progression of the disease, the mean gray scale and area density of Ang Ⅱ and VEGF expression in the diabetic group decreased significantly, and the area density value increased significantly Moreover, the area density of VEGF in the captopril intervention group at 8 and 12 weeks was significantly lower than the value at 4 weeks.AngⅡ and VEGF were significantly positively correlated, and the area density of VEGF in the captopril intervention group was significantly negatively correlated with the course of disease.Conclusion Early application of ACEi or AT1R antagonist can improve DR to some extent and play a protective role on retina.

Fusarium, Aspergillus, Candida and Penicillium are the main infectious fungi of fungal keratitis. Different fungi show different pathological damage characteristics in cornea, and their mechanism is still unknown [1]. In this experiment, the above four fungi were selected to investigate the role of adhesion and matrix metalloproteinases (MMPs) in corneal fungal infection.

hematoporphyrin monomethyl ether (HMME) is a new photosensitizer made in China, which has the advantages of strong photosensitization and small toxic and side effects [2]. This experiment observed the distribution characteristics of HMME in rabbit eye tissues, especially the peak and resolution time of drug concentration in retinal pigment epithelium (RPE), choroid and retina, and provided a basis for the future application of HMME in ocular photodynamic therapy.

At present, the main treatment for glaucoma is to reduce intraocular pressure through drugs or surgery. However, simply lowering intraocular pressure is not enough to prevent the progressive damage of optic nerve caused by glaucoma. Other pathological mechanisms may also lead to retinal ganglion cell (RGC) and optic nerve damage. All factors that may cause RGC apoptosis, such as glutamate toxicity, nutritional factor interruption, vascular abnormalities, glial cell activation and nitric oxide toxicity, may be related to glaucoma lesions [1]. Although there is no fully recognized mechanism of glaucoma at present, researchers can verify various retinal and optic nerve protection strategies based on these theories, and then study and select drugs to prevent and delay RGC lesions without necessarily affecting intraocular pressure. The following author only summarizes the meaningful research results in drug treatment strategies, and other possible treatment methods such as gene therapy, nerve transplantation, autoimmunity and vaccine injection will not be described here.

The Chinese Journal of Ophthalmology was founded on October 1, 1950. It was sponsored by the Chinese Medical Association and edited by the Editorial Committee of the Chinese Journal of Ophthalmology. The editorial committee is composed of 61 well-known ophthalmologists in China. The current editor-in-chief Zhao Jialiang and the editorial director Zhao Hongmei. The main readers of this journal are ophthalmologists across the country, and it is publicly distributed at home and abroad. China Standard Issue Number: ISSN0412-4081, CN11-2142/R; Postal Code: 2-60; National map code: BM93. The Chinese Journal of Ophthalmology has always implemented the principles and policies of the Party and the state on health work, implemented the principle of combining theory with practice, popularization and improvement, reported the major progress and important achievements of clinical and scientific research in ophthalmology in China, and promoted

The Publishing and Distribution Department of Chinese Medical Association Magazine handles the retail/wholesale business of this journal all the year round, and readers can subscribe directly. In 2005, this magazine is a monthly magazine, with a unit price of 16 yuan per volume and an annual price of 192 yuan. Those who subscribe directly for the whole year can enjoy a preferential price of 172.80 yuan. Offer subscription due November 30, 2004. Subscribe at: 010-65249989 ext 1333 or 1332, 65251918. Post office remittance address: Box 58, Dongsi Post Office, Beijing, Postal Code: 100010; Recipient: Publishing and Distribution Department. Please indicate the title, year, issue date and number of copies of the magazine purchased on the remittance document. No postage costs will be waived. If you need to register, you will be charged 2.00 yuan per volume.

Since the change of Chinese Journal of Ophthalmology to a monthly publication in 2002, the number of submissions has gradually increased. In order to meet the needs of readers and authors, the editorial board and the editorial department of this journal jointly negotiated to increase the published length in 2005 on the premise of ensuring the quality of the journal, that is, from 72 pages per issue in 2004 to 96 pages per issue in 2005. After the addition of pages, the magazine will adjust and enrich the columns of this journal, improve the reporting effectiveness, expand the information capacity, and be closer to ophthalmology readers. In 2005, Chinese Journal of Ophthalmology was a large 16-format edition, with 96 pages per issue; Price per volume RMB

Practical Ocular Surface Diseases edited by Professor Zhu Zhizhong has been published by Beijing Science and Technology Press. This book aims at serving the clinical practice of ocular surface diseases, with detailed content, comprehensively discussing the basic theory and clinical knowledge of ocular surface diseases, including the latest progress in theory and technology in the field of ocular surface at home and abroad. This book focuses on the mastery and application of diagnosis and treatment technology, and has reference value for medical treatment, scientific research and teaching of medical staff at all levels of ophthalmology. Address: No.16, Xizhimen South Street, Beijing Science and Technology Press, Postal Code 100035; Contact number: 66161952, 66137380.

The Strabismus and Pediatric Ophthalmology Group of Ophthalmology Branch of Chinese Medical Association will hold the 11th National Academic Conference on Strabismus and Pediatric Ophthalmology in Shenzhen, Guangdong Province from April 22 to 26, 2005. Ophthalmology colleagues all over the country are welcome to submit enthusiastically, and the selected papers will be exchanged during the conference. This conference will give national continuing education credits and Chinese Medical Association paper certificates. Contents: Clinical and basic research on strabismus and amblyopia, clinical and basic research papers on childhood refractive error, cataract, glaucoma, ocular trauma, fundus diseases, ocular tumors, hereditary eye diseases and other childhood eye diseases. Essay requirements: Abstract and full text of about 500 words. The content of the abstract should include the purpose of the study,

The book "Practical Guide for Minimal Surgical Treatment of Retinal Detachment" was written by Professor Ingrid Kreissig of Germany, translated by Professor Hui Yannian of Xijing Hospital of the Fourth Military Medical University, and published by Beijing Science and Technology Press. This book analyzes diagnostic methods, differential diagnosis, and approaches to minimal extraocular surgery in the treatment of retinal detachment, using a conversational narrative to encourage original thinking. Comes with 409 illustrations to provide hands-on instruction in manual form. Volume 1: Diagnosis, interrupted external pad pressure without drainage, and case reports; Volume 2:

Ophthalmology Department of Yantai Development Zone Hospital has advanced ophthalmic medical equipment, and was approved as Yantai Eye Hospital by Yantai Municipal Health Bureau in April 2001. Now we are looking for one deputy director and one attending physician who are skilled in cataract phacoemulsification and eye refractive surgery techniques. Interested candidates should send their resume, standard photos and specific requirements to the office of Yantai Eye Hospital (No.11 Taishan Road, Yantai Development Zone), Postal Code 264006; Contact: Wenfang Song; Contact number: 0535-6372230, fax: 0535-6370606.

Choroidal hemangioma is a benign tumor developed on the basis of congenital vascular dysplasia, which can appear isolated in the posterior pole of the eye fundus or diffusely invade most of the choroid. Its treatment methods include microwave therapy, external radiotherapy, laser photocoagulation, etc. Recently, a case of solitary choroidal hemangioma was successfully treated by transpupillary thermotherapy (TTT) in our hospital, which is reported as follows.

With the development of the times, biomedical health science and technology workers, clinicians and medical editors pay more and more attention to the scientific and rigorous nature of scientific research. In recent years, the author looked through some academic journals in ophthalmology and found that there are indeed some problems in scientific research design and statistical analysis in their papers. In order to effectively improve the level and quality of scientific research design and statistical analysis in ophthalmology, the author expounded the essentials of reasonable choice of scientific research design and statistical method, and analyzed and explained the doubts in detail according to the problems reflected in ophthalmology papers.

Professor Yin Suyun, Honorary Editorial Member of Chinese Journal of Ophthalmology, died in Beijing on August 17, 2004 at the age of 81 due to ineffective treatment. Professor Yin Suyun was born in 1923 in Xuzhou, Jiangsu Province. He graduated from Nanjing Central University School of Medicine in 1948 and stayed at the school as a teaching assistant and resident physician in ophthalmology. After the liberation of Nanjing in April 1949, he worked as a teaching assistant and attending physician in the Department of Ophthalmology, School of Medicine, Nanjing University. Subsequently, he attended the Third Military Medical College, the Fifth Military Medical University, the Fourth Military Medical University, the General Hospital of the People's Liberation Army and the Military Medical College