2015年 · 第38卷第06期

Biofilms attach to various environmental surfaces^[1]Especially the surfaces of medical devices and catheters, leading to recurrent episodes of multiple chronic and refractory infections in clinical practice^[2]。 According to the National Institutes of Health, 80% of human microbial infections are caused by biofilms. The growth of bacteria, the expression of specific genes, and the production of proteins are altered after the formation of biofilms^[3,4]The resistance to antibiotics can be 10 to 1 000 times higher than that of planktonic bacteria^[5]。

Cellular alloantibodies can cause blood transfusion reaction and hemolytic disease in neonates in clinical practice, and in severe cases, they can affect the life safety of patients^[1,2,3]However, the clinical identification of erythrocyte alloantibodies generally adopts the traditional test tube method, which is cumbersome and time-consuming, and requires professionals familiar with immunohematology. Therefore, it is particularly important to establish a simple, fast and specific laboratory identification method. In this study, the application value of microcolumn glass bead method and classical test tube method in the identification of erythrocyte allogeneic antibodies was discussed.