1985年 · 第05卷第03期

The binding of fluorescein isothiocyanate (FITC) conjugated lectins to mucin in benign and malignant colon tissues were studied by using fluorescence microscopy. In colorectal carcinoma, peanut agglutinin (PNA) bound to all 25 cases, while dolicho biflorus agglutinin (DBA) only bound to 7 of 25 cases. By contrast, DBA bound to the goblet mucin of all 25 normal colonic mucosa, but PNA was not. Meanwhile, bauhinia purpura agglutinin (BPA) bound to the mucin either from normal mucosa or produced by colonic adenocarcinoma. Mucin from transitional mucosa and villous adenomas which could be considered as premalignant lesions of colon had an increased positive rate of PNA binding and decreased binding percentage of DBA. These results indicate that an exposed carbohydrate structure Gal β¹一² GalNAc is expressed in the mucin produced by colonic adenocarcinoma which is not normally present in human colon trissue. It may be of benefit to the diagnosis of colorectal carcinoma.

Pathologic diagnoses of 2010 biopsies by fibercolonoscopy were analysed. A variety of diseases in large intestine included inflammation (70.8%) , polyp (13.4%) , cancer (3.9%) and amyloidosis (0.2%) . Ulcerative colitis was ficquently seen in the inflammatory diseases (11.3%) and adenoma was the most common in polyp (64.8%) . All these diseases could be identified by biopsies obtained from fibercolonoscopy, so it is an ideal and effective approach for the diagnosis of these diseases. The criteria of pathologic diagnosis and differential diagnosis of various diseases in large intestine are discussed.

The purpose of this study is to compare motility patterns of rectum and sigmoid in normal subjects with those in patients with the irritable bowel syndrome. In this study, a newly designed type of manometric instrument (Xin-hua ARM-1) was used. Motility index, ano-rectal relaxation reflex and the resting pressure were recorded in both groups. Motility indexes as measured and calculated accord to Connell's method were 0.35 ±0.30 (rectum) and 0.60 ±0.50 (sigmoid) in 16 patients with the irritable bowel syndrome, in contrast to 0.18 ±0.15 and 0.31±0.12 respectively in 18 normal subjects (p<0.05) . A test of ano-rectal relaxation reflex showed no significant difference between normal subjects and patients. Likewise, the resting pressure did not differ significantly in both groups. Our study comfirms that the irritable bowel syndrome is associated with an abnormality in colonic motor function. The measurement of colonic motility index may be benificial to the diagnosis of irritable bowel syndrome. The possible relationship between changes in colonic motility and the irritable bowel syndrome is briefly discussed.

This paper gives an account of using heptafluorobutyric acid anhydride as a derivatizing agent for quantitative estimation of bile acids in human fluids and gallstones. We reduced several derivatizing steps to one, and eliminated the explosive and carcinogenic diazomethane as one of the derivatizing agents. The linearity of determination was found in the range of 0.1-5 microgram. The fasting serum bile acids in 74 patients with acute, chronic hepatitis, liver cirrhosis, cholestasis, late schistosomiasis, and gallstones and biles in 5 patients were determined. This method was found satisfactory to estimate the bile acids in human fluids and gallostones.

Twenty-eight cases of pathologically proved hepatocellular carcinoma (HCC) with erythrocytosis during 1974-1982are reported. The incidence of erythrocytosis in HCC was 7.6%. Erythrocyte count and hemoglobin level ranged from 5.5 to 7.6 x 10⁶/mm³ and 16.5 to 26 g/dl, respectively. All cases were male. Among them 7 cases were found in subclinical stage, amounted to 9% of the overall subclinical HCC. The ertyhrocyte count tended to increase as the advance of the disease. This syndrome disappeared after resection of the tumor in 6 cases. Thus, serial monitoring of erythrocyte count: and hemoglobin level in HCC patient with erythrocytosis may be helpful to observe the response of treatment.

Twenty-six cases of pathologically proved non-Hodgkin lymphoma of the small intestine are reported. Twelve cases (49.9%) were misdiagnosed. Preoperative roentgenologic diagnoses were lymphoma or other malignancies in 18 (69.5%) . At present, barium meal or small intestine barium enema remains to be the principal method for the diagnosis of the small intestine lymphoma. Among the 24 cases treated by various methods, the 5 and 10 year survival rates were 20.8% and 12.5% respectively. Upon analysis it was shown that those in whom radical resection could be done and those suffering from single lesion, seen at the stage I and II, of the B-cell type lymphoma and those treated properly had relatively better prognosis. It was also found that the sites of the neoplasm and the presence of complications did not affect the prognosis.

This paper summerizs the laparoscopic examinations in 136 patients (98 female and 38 male) with intractable chronic chronic abdominal pain. Most of them were under the age of 39, the incidence was found higher in young adults. The course of abdominal pain was mostly over one year, 50% of the cases over 2 years and the longest period even lasted 11 years. Lapaarscopic examinations revealed that abdominal pain was associ ated with adhesion to the local tissues. Among the 113 cases, the adhesion of liver, gall-bladder, abdominal wall, gastrointestine and spleen to their surrounding tissues was found in 75 cases (55,2%) , 18 (12,3%) , 6 (4,4%) and 2 (1,5%) respectively. Abdominalgia disappeared immediately after the separation of adhesion in 4 cases. The author suggests that the condition may be termed as "upper abdominal adhesion syndrome"