1993年 · 第106卷第02期

The persistence of anti-HBs was investigated in 95 juveniles who received plasma-derived HBV vaccine (vaccine group) and 63 counterparts who got anti-HBs from natural HBV infection (infection group) for a period of five years. The positive rates of anti-HBs from the first to fifth year in the vaccine group are 97.89%, 98.95%, 81.05%, 78.95% and 72.63% respectively with one recipient remaining anti-HBs negative after being immunized with three dosages of 10 micrograms plasma-derived HBV vaccine in 0, 1st, 2nd month and the mean S/N values (GMV) are 30.94, 22.18, 13.61, 12.02 and 9.18 respectively. There are 26 recipients whose anti-HBs turned from positive to negative at the end of the study with a negative rate of 27.37%. The S/N GMVs in the infection group are 36.37, 27.33, 24.08 at the first, third and fifth year of the study, respectively. Both the S/N GMV and negative rate are lower than that of the vaccine group (P<0.01). No one was found to have positive HBsAg or elevated ALT in both groups. Questions such as immune dosage, immune program and booster immunization in juvenile population are discussed.

The in vitro anti-viral activity of garlic extract (GE) on human cytomegalovirus (HCMV) was evaluated by tissue culture, plaque reduction and early antigen assay. A dose dependent inhibitory effect of GE was evident when GE was applied simultaneously with HCMV. But the effect was stronger when the monolayers were pretreated with GE. In addition, the anti-viral effect of GE persisted long in infected cells after its being removed from the culture medium. The strongest anti-viral effect of GE was demonstrated when it was applied continuously. It is therefore recommended that clinical use of GE against HCMV infection should be persistent and the prophylactic use of GE is preferable in immunocompromised patients.

In situ hybridization using biotinylated DNA probes has become an important tool in histopathology. It is well known that the sensitivity of the methods used to demonstrate viral DNA in formalin-fixed and paraffin-embedded specimen depends strongly on the detection system used. In the present study, an optimized in situ DNA hybridization protocol was combined with four different approaches of gold-silver staining methods. For silver enhancement, the recently described method of silver acetate autometallography, a technique allowing highly efficient development without the necessity of dark room illumination has been used. The most efficient detection method found in our experiments was the use of gold-adsorbed anti-biotin antibodies with subsequent silver enhancement. This staining procedure can be completed in 5 hours including hybridization and is a highly sensitive alternative to peroxidase and alkaline phosphatase detection systems.

Sections from 88 paraffin-embedded tissues identified histopathologically as condyloma acuminata were assayed for the presence of human papillomavirus (HPV) types 6, 11, 16, 18 and 33 using polymerase chain reaction followed by specific hybridization. HPV DNA sequences were found in all tissues of condyloma acuminata. HPV type 6 was present in 71 tissues, type 11 in 16, and type 16 in 2. One man was doubly infected with HPV types 6 and 16. No HPV types 18 and 33 were found. We conclude that condyloma acuminata is mainly produced by HPV types 6 and 11.

The cellular DNA and RNA contents of trophoblastic tissue of 86 patients with trophoblastic disease and chorionic tissue of 10 patients in early pregnancy were analysed by flow cytometry (FCM) and strict follow-up was carried out. The result showed the rate of accurate diagnosis by DNA and RNA determinations were 60.0% and 85.7% respectively in benign moles; 86.7% and 86.7% in invasive moles; and 100% and 100% in choriocarcinoma. Statistically the pseudopositive percentage of RNA was clearly lower than that of DNA (0.01<P<0.05). In addition, the trophoblastic cells in curetted molar tissue and the uterine tissue later proved by surgery of 10 invasive moles were simultaneously assayed for comparing their DNA and RNA contents, showing statistical significance (P<0.001). The DNA and RNA contents in different cellular cycles were analysed by FCM and there was no statistical significance among benign, malignant moles and choriocarcinoma (P>0.05). Our study suggests that aneuploidy DNA content may be an indicator for malignant tendency and the RNA parameter rather than DNA is more sensitive for the prognosis of hydatidiform mole.

This paper typed HLA-A, B, C and DR antigens in patients with psoriasis vulgaris and healthy persons in 12 families. The phenotype frequencies of HLA-A1, B13, B17, Cw4 and Cw6 in the patients were higher than those in the controls. The analysis of haplotype segregation showed that HLA haplotypes in families with more psoriatic cases were marked deviation from random distribution. One or two HLA haplotypes sharing among affected siblings occurred more frequently than expected. The result of Lods score for six families showed that there was a close linkage between the gene of psoriasis vulgaris and HLA antigens. The gene of psoriasis was coincided with the recessive hereditary mode.

Three pairs of human cadaver femora were used for strain measurement under a weight-bearing simulation test, which was performed on intact bone and osteotomized bone fixed by 3 types of plates with different rigidities respectively. The plates with uniform thickness did not adhere to the uniform stress distribution along the plate. The stress between both central screw holes was significantly great. However, the device of trapezoidal plate conformed to the uniform stress distribution along it, and the safety factors of 3 different types of the plates were proposed. The osteotomy site was mainly subjected to compression stress in standing phase with load on both femora, but subjected to bending stress mostly with load on the single femur. The total compression strain at the osteotomy site was greater than that on intact bone while the load was adding over twice body weight with load on both legs, and nearly normal with load on one leg. We believe that the bone structural changes caused by stress shielding and limb disuse etc can be overcome by using optimal plate and normal weight bearing.

The definitive diagnosis and determination of recurrence of herpes simplex keratitis are still difficult in clinical ophthalmology. At present, isolation of virus by tissue culture is perhaps the best method for establishing a specific aetiological diagnosis of viral infection. But due to its complicated and time-consuming procedures, the application of tissue culture for virus isolation in clinical work is still limited. In situ DNA hybridization is a specific and quick technique for directly detecting genetic materials, DNA and RNA, of viruses. In this study, this technique was used to identify herpes simplex virus type 1 from a patient's cornea suffered from recurrent herpetic keratitis. The technique offers a convenient and specific method for clinicians to make a definitive diagnosis and differential diagnosis of viral infectious diseases. The advantages and disadvantages of other different methods available for viral diagnosis, such as light and electron microscopy and immunohistochemistry were discussed with an emphasis on in situ DNA hybridization.

Serum copper and zinc were simultaneously determined by ion-exchange chromatography (IC) in 35 normal volunteers and 45 patients with benign diseases or different stage cancers of digestive tract. No significant differences of serum Cu, Zn (both expressed in μmol/L) and Cu/Zn ratio were found among normal volunteers, patients with benign diseases and those with stage I or II cancer. While in the group of stage III and IV cancer patients, the mean of serum Cu (26.5±1.4 μmol/L) and Cu/Zn ratio (2.25±0.16) increased significantly (P<0.01) and that of serum Zn (11.8±0.5 μmol/L) decreased markedly (P<0.05). Our results showed that serum Cu, Zn and Cu/Zn ratio was of no help in detecting early stage cancers and in differentiating them from benign diseases but was useful in predicting stages of cancers before operation for a better choice of therapy.

Isolated live hair cells are important models for studies on the electrophysiology, pathology and pharmacology of the hair cell. Using mechanical isolation method after papain treatment, 70±27 hair cells, including 0 to 4 inner hair cells, could be obtained from each cochlea of 4 guinea pigs. The criteria for a good viability of isolated cochlear hair cells were: 1) a smooth hair cell membrane; 2) hair cells not swollen; 3) the nucleus in normal position; 4) the cytoplasm in a state of translucence with a halo at the periphery (birefringence); and 5) no Brownian movement of the organelles within the cytoplasm. With short-term culturing at room temperature, approximately 90% of the isolated hair cells retained a good viability at the end of two hours. Subsequently, the hair cells gradually degenerated but still, at the end of five hours, about 40% of them appeared intact. The degeneration patterns have been carefully observed and described.

This paper deals with the ultrasonic studies of the effect of artemisia decoction (AD) on the volume and motion of gallbladder in 33 cases. Ultrasonic examination shows that AD intravenous infusion has remarkable effects on the contractility of gallbladder. There are 4 patterns of phasic changes in the motion of gallbladder and an increase in frequency of its contraction and relaxation. AD has also certain contraction effects on the gallbladders which can not contract after a fatty meal. The above findings indicate that AD is a good choleretic and has a definite regulating effect on the motility of the gallbladder. The clinical use of AD is conducive to bile flow, stone expelling, inhibiting the deposition of bile solids and reducing the possibility of stone formation.

A sporadic case of Hallervorden-Spatz Syndrome was diagnosed before death. The diagnosis was made on clinical manifestations and histopathology of the tissue fragments obtained from the globus pallidus by stereotaxic brain biopsy. The involvement of thoracolumbar spinal cord is discussed. The cranial computed tomography showed caudate atrophy with bicaudate index (CC/OTcc) of 0.2, and the quotient FH/CC of 1.43, which were indistinguishable from Huntington's disease.