The limited potential for proliferation of human fibroblasts in culture represents cell level senescence. Aging is a programmed process under genetic control, and at certain stage of the life-span of animal cells, some genes start to express. Studying the biomarkers of senescent cells is important to understanding the basic mechanism of aging which may be relevant to the normal cell growth control and tumor biology. A protein with 72,000 Dalton molecular weight was detected by the hybridoma method in our laboratory. The protein shows specificity to senescent or presenescent cells of several cell lines, including WI-38, K.D., U2OS, etc.
We studied 4 cases of quadrilateral space syndrome. Though the syndrome is quite uncommon, it might be discovered by examination of the patient complaining of pain of neck and shoulder especially when the tenderness in the quadrilateral space. Early surgical decompression is indicated as soon as diagnosis is made.
Cao Xicai, Wu Enhui, Chang Jiwu, He Nengshu, Bai Jingwen, Yang Haixian
CHINESE MEDICAL JOURNAL1995年 108卷 02期
DOI: 10.3760/cma.j.issn.0366-6999.1995.02.112
摘要
The mechanism of percutaneous transluminal renal angioplasty (PTRA) was studied in 18 dogs. The dogs were divided into two groups. Seven dogs were in the early group and eleven were within 1-3 weeks after PTRA. A 4/0 resorbable chronic catgut was used to ligate subtotally the renal artery to create fibromuscular dysplasia. PTRA was performed after renal artery stenosis for 6-8 weeks. The changes of ultrastructure of renal artery were studied. A contrary orientation balance hypothesis was proposed to explain the mechanism of PTRA.
Thirteen cases of intramedullary hemangioblastoma of the spinal cord were studied radiologically, especially with magnetic resonance imaging (MRI). Microsurgery was used to remove the tumors in all cases. Signs and symptoms were improved in 84.6% of the cases after operation. The diagnosis and microsurgical techniques for excision of intramedullary hemangioblastoma of the spinal cord are discussed. This disease should be differentiated from hydromyelia, intramedullary ependymoma, and vascular malformation of the spinal cord. Total removal of the tumor is recommended.
We reviewed retrospectively 139 patients with squamous cell carcinoma of the maxillary sinus who were treated with planned combinations of preoperative radiotherapy and maxillectomy between 1958 and 1987. The 5-year survival rate of these patients was 30%. In 111 patients who had tumor invasion of the orbital floor 88 underwent radical surgery with orbital exenteration and 23 had their orbital contents preserved. If orbital exenteration was performed, the 5-year survival rate was 27.3% (24/88) and the rate of recurrence within the orbit was 12.5%. In patients with preservation of the orbital contents, the corresponding rates were 34.8% (8/23) and 8.6% respectively (P=0.65). Histological study after maxillectomy with orbital exenteration showed that in 95% of 65 specimens (62/65) invasion of the orbit was limited. These tumors could be completely resected by total maxillectomy with limited removal of the periorbital tissues after preoperative radiotherapy. We conclude that after preoperative radiation, the eyeball could be safely preserved in most selected patients with maxillary sinus carcinoma invading the orbital floor.
Li Derong, Huang Rutong, Tian Xing, Yin Shurong, Wei Jun, Huang Xianrui, Wang Baozhen, Li Ruixia, Li Yuchuan
CHINESE MEDICAL JOURNAL1995年 108卷 02期
DOI: 10.3760/cma.j.issn.0366-6999.1995.02.115
摘要
The morphology and morphogenesis of isolated hepatitis E virus (HEV, strain 87A) were observed by electron microscopy (EM) and immune electron microscopy (IEM). Progressively developing local vesicles, virions accumulation in crystalline arrays and viroplasmic focus were seen in cytoplasm of infected cells. Replication and assembly of the new generation viruses were closely associated with rough endoplasmic reticulum (RER), inclusion body (IB) and microfibrils. Condensation and margination of chromatin, dispersion of nucleolar material, nuclear membrane alteration and masses of threads, granular material, and fibrillar component of the nucleus were frequently found. These changes revealed that this strain virus was confirmed as a RNA virus. The shape of the virus particles appeared approximately spherical whether the specimens were from the tissue culture crude suspension or purified highly concentrated preparations. The size of the virion was about 30 nm in diameter. The viral particles appeared unsmooth and irregular in outline. The spike-like structures may be occasionally observed on the surface of some viral capsides. The diameter of the strain 87 A virus is larger than the picornavirus and smaller than the calicivirus. This strain virus is different from classical calicivirus in without the cup-shaped surface depressions. The new genus, heparnavirus genus of caliciviridae family should be proposed for HEV.
The argyrophilic staining technique was used in differential diagnosis between non-Hodgkin's lymphoma (NHL) and reactive hyperplasia (RH) of lymphatic tissues in 30 cases of RH and 71 cases of NHL which were classified as low (11 cases), moderate (49 cases) and high malignant (11 cases) groups. The number and the area of Ag nucleolar organizer region (AgNORs) in 100 cells of each case were examined by both IBAS-200 cytoimage analyser and microscope. The results showed that there was significant difference in the number, but not in area, of AgNOR granules between RH and low malignant group of NHL (P<0.001). In NHL, the significant differences in the number and area of AgNORs were found between each groups (P<0.05 or P<0.001). It was also observed that in NHL, the number of AgNOR was negatively correlated with the area of the granules, and the shapes and distribution patterns of AgNOR were significantly different between each groups. Our results suggest that the quantitative study of AgNOR combining with the histological characteristics is very helpful in the differential diagnosis of RH and NHL and in the classification of NHL.
During December 1989 to December 1992, conjunctival specimens from 63 patients with severe trachoma and 30 with acute follicular conjunctivitis at the eye clinic of Tong Ren Hospital in Beijing, were tested by using direct immunofluorescent technique and amplified enzyme-linked immunoassay (Micro Trak and IDEIA) for chlamydial antigenicity. Trachoma accounted for 97.6% while acute follicular conjunctivitis accounted for 2.4% of the positive cases. Micro Trak and IDEIA tests proved advantageous over the culture method for the detection of Chlamydia trachomatis. In our study, Ofloxacin eye ointment has been proved to be an alternative drug for severe trachoma with better curative effectiveness.
The content of lymphocytic and erythrocytic K+, Na+, Ca++, Mg++ changed in patients with congestive heart failure, related to clinical treatment and prognosis. The longer the course of disease, the more significant the changes, with no relation to the etiology of heart disease. The changes were partially improved after treatment of the heart failure. The content of electrolytes is not significantly different in patients in NYHA Class III, compared to those in NYHA Class IV. The metabolic changes of electrolytes occur on the myocardial cells, skeletal muscle cells and peripheral erythrocytes in patients with congestive heart failure. However, serum electrolyte content does not reflect precisely overall electrolyte metabolism in the body. Whether similar changes of electrolyte content occur in the peripheral lymphocytes is rarely reported. We studied the lymphocytic and erythrocytic electrolyte content in patients with congestive heart failure, to provide some reference in the clinical treatment and prognosis of congestive heart failure.
In this study the effect of Sheng-Mai Injection i.e. Red Ginseng-Ophiopogon Root Injection (one kind of traditional Chinese medicines) on the contractivity of diaphragm was observed. The results confirmed that Sheng-Mai Injection increased Pdi of the fatigued diaphragm in rabbits and reduced the time needed for the recovery of Pdi of fatigued diaphragm to the normal value. These results suggest that Sheng-Mai Injection can increase the contractive force and promote the recovery of the fatigued diaphragm. The effect of Sheng-Mai Injection on the contractivity of the isolated diaphragmatic bundle of rats was also observed and the results confirmed that Sheng-Mai Injection increased the diaphragmatic contractive force directly. This effect of increasing the contractive force of diaphragm was attenuated by adding calcium channel blocker isoptin and disappeared when there was no calcium in the extracellular fluid. It is deduced, therefore, that the mechanism of the effect of Sheng-mai Injection is related to the increased influx of calcium from extracellular fluid into the cells.
Resolution on the prevention and control of diabetes was adopted by the World Health Assembly (WHA) in 1989, which indicates that diabetes severely threatens the health of people. In order to carry out the resolution, WHO Study Group Meeting on Prevention of Diabetes was held in Geneva, 16-20 November, 1992. I attended this meeting and will discuss my experience as follows.
