1988年 · 第101卷第09期

Campylobacter jejuni, strain CJ-S131, could persistently colonize the murine gastrointestinal tract, inducing extraintestinal inflammation other than chronic intestinal inflammation. In this study, we have investigated the histological changes in the kidneys of the mice (BALB/c and KM) infected with CJ-S131 bacteria by light microscopy (LM), electron microscopy and histoimmunofluorescent techniques, and the serum isotypical antibody to outer membrane proteins (OMP) of CJ-S131 and the specific circulating immune complexes by ELISA. The results demonstratcd that there was post-infectious nephritis in the mice which had been once orally challenged with CJ-S131 three months before, as characterized by (1) proliferation of mesangial cells; (2)depositing of the immune complexes, predominantly with IgA in the glomeruli and on the wall of the renal arterioles and venules, consisting of CJ-S131 specific antigens and antibodies; (3) monocyte infiltration and ten dency of fibrosis in the glomeruli; (4) tubulointerstitial inflammation with focal plasmablast infiltrations; and (5) periarteritis in the kidneys. In addition, the protein casts could be found under LM. In the serum, IgA, IgG and IgM isotype antibodies to OMP of CJ-S131 were significantly increased; accordingly, CJ-S131 specific circulating immune complexes appeared in the serum, especially in the KM mice isotype antibody of IgA was significantly higher than IgG. The results suggested that chronic intestinal infection by C. jejuni could induce nephritis, especially IgA immune complex glomerulonephritis, and KM mice were more susceptible to the post-infectious nephritis.

Autologous adrenal medullary tissue was transplanted into the caudate in eleven patients with severe parkinsonism, of whom 8 were male and 3 female, with age ranging from 46 to 68 years. The duration of their illness varied from 4 to 16 years. All patients showed rigidity, bradykinesia, resting tremor and postural abnormality in late stage. Before transplantation, the patients failed at medication of L-dopa combined with amantadine, artane or bromocriptine, but after transplantation, the rigidity of body and motor disturbance were improved significantly and tremor was improved moderately. The techniques used in transplantation were modified: the amount of tissue transplanted averaging 40 pieces; the interval between adrenalectomy and intracaudate implantation within 20 minutes; and the tissue bathed by cooling nourished fluid (4C) before implantation. Functional improvement and indications for intracaudate transplantation, relations between the amount of tissue pieces changes of dopamine level in CSF, and improvement of clinical manifestation are discussed.

From 1949 through 1985, 44 patients with liver metastasis were found in a total of 696 patients with choriocarcinoma admitted to our hospital, giving an incidence rate of 6.3%. Liver metastasis was preceded invariably by pulmonary metastasis in all 44 patients, and coexistent general metastases, particularly those to the spleen and bowel, were demonstrated in most of the patients. Thus, two possible routes of metastasis to the liver are suggested: the involvement of the liver by the general circulation after pulmonary metastasis; and secondary involvement of the liver to intestinal and splenic metastases through the portal system. Chemotherapy, systemic and/or regional, has been the treatment of choice for liver metastasis since 1965. In systemic chemotherapy, combined 5 fluorouracil (5FU) and kengshengmycin (KSM) was given by intravenous drip, while in regional chemotherapy intrahepatic arterial infusion of 5FU and/or methotrexate (MTX) was used by retrograde catheterization through the right epiploic artery. The survival rate for the patients was raised from zero t0 23.3%. In early detection of liver metastasis for a good prognosis, isotope scan and ultrasonography have been considered useful procedures, in addition to routine exploration of viscera in the upper abdomen, the liver for instance, during pelvic surgery for unsuspected cases.

The anterior crucial ligament and medial collateral ligament of the left knee joint in 32 New Zealand rabbits were sectioned. The resulting joint in stability led to osteoarthritis of the knee joint. Trans mission electron microscopic study revealed that during the course of development of osteoarthritis there were changes in the part of both the articular cartilage matrix and the cells. The matrical changes comprised those in size and orientation of the collagen fibrils and in proteoglycans. The cellular changes involved proliferation, degeneration and those in configuration of the cells. AlI these changes varied with different localities of the articular cartilage and with the time of articular instability.

The culture of endothelial cells derived from isolated Wistar rat cerebral microvessels has been accomplished in our laboratory. The microvessels were isolated by a mechanical dispersion and filtration technique. Under phase contrast microscope, isolated microvessels were either in single piece or in tree-branch shape. Collagenase-treated microvessels were seeded with 2.5 ml medium 199 into culture flask at a density of of 10-30/cm2 and incubated at 37℃ in the 5% CO2 air. After 1 or 2 days, cells were seen moving out from the microvessels. On the 3rd or 4th day, many cell colonies were found. Migration continued and marked proliferation began at 5 to 7 days and lasted to 12 to 14 days. In about 2 weeks, the cells were in monolayer, and flat and polygonal in shape. Finally the cells assumed a typical cobblestone appearance. Their nuclei were round or oval, and each had one or two nucleoli. The endothelial origin of these cells was demonstrated by indirect immunofluorescent staining for factor VIII antigen. Ultrastructural examination of endothelial cell cultures revealed that mitochondria, pinocytotic vesicles, lysosomes and rough endoplasmic reticula were rich in plasma and Golgi complex was frequently seen. But no Weibel-Palade bodies were detected in this culture. The population doubling time was 72 hours in passage 3.

Fifty patients with primary hepatocellular carcinoma (PHC) and 49 controls were assayed for hepatitis B virus (HBV) markers by radioimmunoassay. The results showed that the hepatitis B surface antigen (HBsAg) was positive in 43 out of 50 (86%) PHC patients and in 11 out of 49 (22.5%) controls (p<0.001). Both anti-HBs and anti-HBc were present in six PHC patients and in 27 controls. The positive rate for at least one HBV marker was 98% (49/50) in the PHC patients. The relationship between HBsAg positivity and PHC in our young patients suggests that these individuals were infected by the HBV in their early life either by vertical transmission from the carrier mothers during the perinatal period or by close family contacts during the postnatal period. This study confirmed the strong geographic correlation between HBsAg prevalence and incidence of PHC.

The physiologic variations of serum lipids in the residents in urban districts of Beijing from birth to senescence were studied by standardized methods. 15 208 were profiled for serum total cholesterol and 5 585 for high-density lipoprotein cholesterol and triglyceride. The average serum lipids of neonates were similar to those reported by other authors over the world. The characteristic sex and age differences of total and low-density lipoprotein cholesterol showed a striking similarity to those reported by the Lipid Research Clinic, USA, but the mean values were much lower in Beijing residents. However, Beijing boys and men had higher high-density lipoprotein cholesterol than American counterparts. The cholesterol and triglyceride reached top levels at age 60-80 and declined after 80. The nonagenarians had similar lipid levels as middle-aged adults. The definition of hypercholesterolemia was discussed briefly. The lipid risk level for coronary heart disease of Beijing populations was estimated.

Human peripheral blood T helper cells (Th, Leu 3a+ cells) and T suppressor cells (Ts, Leu 2a+ cells) in 26 cases of systemic lupus erythematosus (SLE) and 13 normal controls were studied by a two-colour monoclonal antibodies (Becton Dickinson, U.S.A.) direct immunofluorescence method. Three of 26 SLE patients were followed up for two months after treatment. It was found that the Th/Ts ratio in normal controls was l.6±0.6 (1.0-2.2), whereas the ratio in 73% of SLE patients was abnormal, with either Th or Ts deficient. All these cases had clinical symptoms of moderate or severe degree, including involvement of the key organs. SLE patients with a normal Th/Ts ratio were in mildly active or inactive stage of the disease. Treated with prednisone and/or cyclophosphamide the Th/Ts ratios in active SLE patients were reduced as a result of the increase of Ts cells.

Rabbit antiserum to HBxAg and PAP technique were used to detect HBxAg in liver tissue of 150 patients with chronic liver disease. HBsAg and HBcAg were also localized at the same time. Positive granules for HBxAg were localized in some liver cell nuclei and no cytoplasmic HBxAg was seen. The incidence of HBxAg was 12.5% in 80 patients with viral hepatitis and 5% in 20 patients with cirrhosis. HBxAg could not be detected in 50 patients with HCC. HBxAg was only expressed in the liver tissue of cases positive for HBsAg and/or HBcAg. There was no relationship between expression of HBxAg and histopathological changes.

On the basis of our results, we believe that the hamster can be used in the study of epidemic hemorrhagic fever virus (EHFV) for isolation and propagation of EHFV, production of immune serum and antigen slides, as an animal model for drug screening and vaccine testing, etc.